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Lysates were sonicated 3X 10 seconds, centrifuged at 10,000 rpm for 10 minutes and incubated with anti-FLAG M2 affinity gel (A2220, Sigma Aldrich) rotating overnight at 4C to immunoprecipitate the SNAP/FLAG-hGLP-1R
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Retrieved 5 April 2024
This paper was published in Frontiers in Nutrition and was the result of the one-year experience of 50 patients, taking part in a metabolic health and weight loss clinic
The study identified a specific communication pathway within the cells, known as the Wnt pathway, which is activated by semaglutide to produce these bone-strengthening effects
