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INS-1(832/13) cells were cultured in RPMI-1640 medium supplemented with 10 mM HEPES, 10% heat-inactivated FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.05 mM -mercaptoethanol, 100 iU/ml penicillin, and 100 g/ml streptomycin at 37 C in a humidified 5% CO 2 atmosphere

Why Trust Everyday Health Caitlin Beale, MS, RDN Author Caitlin Beale, MS, RDN, is a registered dietitian and freelance health writer with experience in clinical nutrition, education, research, and private practice

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