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The necessity for apical glucose transport is demonstrated in Sglt1 / mice, where glucose-stimulated GIP secretion is eliminated and levels rise only to the same extent as observed in the saline control (Gorboulev et al., 2012)

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Dual luciferase activity was measured using a GloMax 20/20 luminometer (Promega) following sequential reagent addition: firefly luciferase activity was recorded first, after which the reaction mix was supplemented with stop solution and Renilla luciferase substrate to initiate the secondary reaction