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The samples were centrifuged (10,000 g , 10 min) and 20 L of the supernatants plus 60 L of 0.66 mg/mL DTNB solution, 60 L of 0.66 mg/mL -NADPH (Sigma) and 60 L of 3.33 units/mL glutathione reductase (Sigma) were used for DTNB assay

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