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GST pulldown assay The prey proteins (PIF4, PIF4 mTAD , PIF4 mB , and PIF4 mBH ) were synthesized in vitro using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc., Ipswich, MA) according to the manufacturers instructions

For the C5 domain of NocB a unique amino acid located immediately upstream to the conserved HHxxxDG motif has been reported, which is crucial for the proper function of C5 29

PMID: 19231263

Many people begin noticing appetite suppression during this phase

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