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Subsequently, 225 L of substrate solution (100 mM Na phosphate, pH 7.0, 20 mM KCl, 1.0 mg mL 1 o -nitrophenyl--D-galactoside, 2.7 L mL 1 -mercaptoethanol) was added to the permeabilized-cell mixture, and the resulting reaction was incubated at 30 C with shaking

screened hub genes based on the weighted correlation network analysis (WGCNA) algorithm and identified interconnected transcription factors (TFs) and non-coding RNAs (ncRNAs) using principal component analysis
doi: 10.1016/j.sleep.2021.02.036 5

Kang M, Yin J, Ma J, Wu X, Huang K, Li T et al
