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The next day, the epidermis was removed and the obtained dermis fragments were placed on 6-well plates in Dulbeccos Modified Eagle Medium with high glucose 4,5 g/L (DMEM HG) (Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich) and 1% P/S (DMEM HG complete medium)

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Follicles pushed from telogen into anagen must first shed their old hair before producing new growth