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A significant moment in the progression of research in this field occurred when researchers used syngeneic transplantation to place pineal glands from young mice into the thymus of older mice, resulting in a prolonged lifespan

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These included measuring the degree of shortening (contracture) of the operated leg compared to the unoperated one, calculating the walking recovery index (WRI) and the motor function index (MFI), and thigh hypotrophy by comparing the muscle diameter of the operated and non-operated side, as well as measuring the distance between the muscle fibers and anatomical insertion sites for the iliac and femoral bones