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The resulting cells were harvested through centrifugation at 800 rpm for 5 min and then washed twice with ice-cold phosphate-buffered saline (PBS)

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Serum semi-target analysis was conducted by 1290 Infinity LC (Agilent Technologies, USA) coupled to a 6490 Triple Quadrupole MS system (Agilent Technologies, USA) in the dynamic multiple reaction monitoring (dMRM) mode