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After 45 min of infection, infected cell monolayers were washed three times with pre-warmed DMEM and further incubated with fresh DMEM containing 10% FBS and 50 g/mL gentamicin at 37 C and 5% CO 2 for 2 h to kill extracellular bacteria

As demonstrated by the chick chorioallantoic membrane (CAM) assay and endothelial tube formation assay, BPC 157 could increase the vessel density both in vivo and in vitro, respectively

Nicastri PL, Diaferia A, Tartagni M, Loizzi P, Fanelli M

Luedike, P

Some nutritionists recommend increasing the intake of the amino acids cysteine and glutamate, which make glutathione, but this hasnt been studied either