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Extrapineal melatonin: analysis of its subcellular distribution and daily fluctuations

doi: 10.1038/s41531-023-00518-9 161 TaipaR.PereiraC.ReisI.AlonsoI.Bastos-LimaA.Melo-PiresM.et al

Inner Mitochondrial Membrane Disruption Links Apoptotic and Agonist-Initiated Phosphatidylserine Externalization in Platelets

Briefly, frozen pellets were resuspended with Milli-Q water and homogenized using a Pellet Pestle Motor (Kimble) for 30 s, alternating with 10 min incubation on ice (repeated for three times)

MTT cell proliferation assay The viability of treated U251 cells was initially assessed in 96-well plates using the colorimetric MTT cell proliferation assay kit, following the supplier instructions
