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The solution was then added to an RNAeasy Mini spin column (Qiagen) placed in a 2 mL collection tube and centrifuged for 15 s at 8000 g at RT

The dietary intake of vitamins and amino acids also helps prevent alcoholic liver damage

Under normal conditions, 5-HT cannot cross the bloodbrain barrier, so increases in its levels in peripheral blood would not increase CNS 5-HT levels [284]