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Protein was induced with 1 mM IPTG after cells reached an absorbance of 0.5 at 595 nm

Conversion of retinaldehyde to retinoic acid is catalyzed by a family of retinaldehyde dehydrogenases (RALDH) that are members of the aldehyde dehydrogenase 1 family of enzymes

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Notably, NAM serves as the substrate for NAD + synthesis in the salvage pathway (Pissios, 2017)

2 References: Hilary J Powers, Riboflavin (vitamin B-2) and health, The American Journal of Clinical Nutrition, Volume 77, Issue 6, June 2003, Pages 13521360, of vitamin B12 and folate deficiency Dutta, P, et al