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Full-length -SNAP (WT and M105I mutant) coding sequences were PCR amplified and cloned in-frame into the pGST-Parallel-1 vector using EcoRI and SalI sites

Macrophages supplemented with glutamine have revealed that stimulation with LPS increased NF-kB activation under 2 and 10 mM of glutamine supplementation in comparison to macrophages treated with 0 and 0.6 mM of glutamine, as examined by DNA binding activity of NF-kB using an electrophoretic mobility shift assay (EMSA) [90]

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Iltaf J, Noreen S, Rehman MFU, Ghumman SA, Batool F, Mehdi M et al (2021) Ficus benghalensis as potential inhibitor of 5-Reductase for Hair Growth Promotion: in Vitro, in Silico, and in vivo evaluation

Figure 3 Bioactive compounds from medicinal plants offer promising solutions to the growing AMR crisis by disrupting microbial proteins and modulating host immune responses