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Furthermore, fatty acid supplementation modified the composition of the GM and enhanced the presence of Akkermansia and Aristochthys spp., both of which were associated with the generation of SCFAs

doi: 10.1371/journal.pone.0073085
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The pre-frozen solution was then subjected to freeze-drying

The sample was vortexed for 30 s and incubated on ice for 30 min followed by incubation at -20 for 2 h
