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Additionally, lactylation, a newly identified post-translational modification associated with lactate metabolism, has been shown to influence protein function and gene expression, further implicating its role in PH

3.3 Alpha-lipoic acid Alpha-lipoic acid (ALA) is a natural compound commonly found in the diet, serving as a crucial cofactor for mitochondrial respiratory enzymes and playing a vital role in maintaining cellular oxidative metabolism (Holmquist et al., 2007)

Subsequently, the supernatant was removed and the cells were washed two times with HBSS, and the working solutions of MitoSOX red (10 M) and Hoechst33342 (1 g/mL) were added, and the cells were further incubated for 30 min at 37 C in the dark

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