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In vivo co-immunoprecipitations Phoenix or HaCaT cells were lysed in lysis buffer (50 mM Tris-Cl, pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1% Triton X-100, 0.1 mM Na 3 VO 4 , 1 mM PMSF, 10 g/ml Leupeptin) for 30 min on ice

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10.3844/ajassp.2010.201.207 51 OhinataY.YamasobaT.SchachtJ.MillerJ

DeepTools: A Flexible Platform for Exploring Deep-Sequencing Data
