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Following treatment with GHK or GHK-PEG, the number of cells per ml of media was assessed as previously described and the rate of cell proliferation was expressed as fold control and compared between treated and non-treated cells using analysis of variance (ANOVA)

Statistical analysis The mean and standard deviation (SD) are shown for the data in each figure, except where n T)GATA mutant mice

IntJ Parasitol

Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat

These studies indicate that gene silencing correlates with DNA methylation, consistent with data from our study, which investigated vitamin D metabolizing gene expression