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Examples include ionizing radiation such as UV light or X-rays, and chemicals such as alkylating agents (e.g
boulardii and L
The proposed pathomechanisms include the hyperglycemia-induced elevated sorbitol and glucosamine pathway, dysregulated glycolysis, and mitochondrial oxidative phosphorylation in diabetic nerve tissues leading to mitochondrial dysfunction and oxidative stress [46]
The luciferase expression was checked at 48 h post transfection by incubating transfected cells with 50 M D-amLu, and the luminescence was recorded by Varioskan LUX multimode microplate reader
