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NG was included to verify that no Asn to isoAsp conversion occurred

Yes, but planning matters

Bioinformatics 25 (16), 20782079 (2009)

The cells were then collected by centrifugation, washed with cold PBS and incubated with annexinV-Pacific Blue in annexin-binding buffer at 20 C for 15 min

Only then can a regulatory submission be prepared followed by an independent review by agencies such as the EMA or FDA
