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The cells were then maintained for at most 6 days with replacement of medium every 2 days

After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

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903 Iron-chelating agents inhibit osteoclast bone resorption and prevent bone loss due to ovariectomy