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In addition, serum levels of IGFBP3 are inversely associated with esophageal and gastrointestinal cancer [6, 7]

With the disease progression, more mesenchymal patterns would be observed

For endogenous immunoprecipitation, the cells were lysed, the lysates were incubated with anti-WDR24 antibody (rabbit IgG used as control) and protein A/G magnetic beads (MCE) overnight at 4 C, washed five times with 0.1% Tween-20 and boiled with SDS loading buffer for 10 min 68

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Increase fiber intake gradually, drink adequate water (at least 8 glasses per day), and consider a magnesium citrate supplement if needed