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The samples were allowed to solubilize for 30 min on ice, and particulate matter was removed by centrifugation at 14,000 g for 15 min at 4 C

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Increased levels of TCA cycle metabolites, including succinate, malate, and citraconate/glutaonate were also identified in the present study (Fig

Enzymatic assay for recombinant human HNMT An enzyme solution (0.1 M TrisHCl buffer [pH 7.4] containing 10 nM rhHNMT [Novus Biologicals, Centennial, CO, USA] and 0.4 mg/mL BSA) and substrate solution (0.1 M TrisHCl buffer [pH 7.4] containing 10 M histamine and 10 M SAM with or without ERGO) were separately preincubated at 37 C for 5 min, and 25 L of each solution was mixed together

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