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Atherosclerotic lesions contain a higher concentration of oxidizable phospholipids and oxidized lipoproteins, have pro-inflammatory and low oxygen conditions (microdomains) which reduce antioxidant availability, and in many of these high-risk plaques there is increased redox active iron accumulation via abnormal iron regulation or heme turnover from bleeding

Journal of Biological Chemistry, 268 (4), 25712576

The gradient started with 5% solvent B (0.1% formic acid, 100% acetonitrile) and increased to 32% over 70 min

We see this play out constantly in our clinic

Long-lived coherences for homogeneous line narrowing in spectroscopy