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showed that L-carnitine reduces serum CRP levels in rodents infected with the SARS-CoV2 virus and in patients with COVID-19 [20]

For intracellular cytokine and MMP-9 detection, PC-3, DU-145, LNCaP, BPH, and TNF (10 ng/mL) pre-activated HUVECs (3 10 5 cells/per FACS tube) were fixed and permeabilized, using the CytoFix/Cytoperm kit (Becton Dickinson), and stained with the following PE-conjugated Mabs: anti-human VEGF (Clone #23410, R&D Systems), CXCL12/SDF-1 (Clone #79018, R&D Systems)

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As a widespread model animal in research areas such as development, molecular genetics, toxicology and biomedicine 26,27 , zebrafish ( Danio rerio ) has been used in mechanistic studies of fish nutrition 28,29