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HDX-MS reveals conformational changes in the C-terminus and PLC core that occur upon membrane binding Coincubation of PLC2 with PS/PE/PIP 2 containing lipid vesicles revealed multiple regions of significantly decreased deuterium exchange (defined as changes greater than both 4% and 0.4 Da at any timepoint with a student t-test value less than p 40% decrease in deuterium incorporation (Figure 3A-D)

[1] [9] [10] [11] [12] They maintain small home ranges around these water sources and spend about a third of their time foraging for resources (especially in regions of abundant leaf litter and dense vegetation), returning to the water for refuge