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In order to construct OXT/OXTR reporter plasmids, the gene promoter (2 kb upstream of the transcription start site plus first exon) was amplified from Ensembl gene ID: OXT-201 ENST00000217386.2 (for OXT) and OXTR-201 ENST00000316793.7 (for OXTR) by PCR and subcloned into the pGL3-basic vector (# E1751, Promega) using underlined restriction sites with the following primers: OXT forward: 5-gcgc- acgcgt - ttg gat gcg ggc cac ctg gga -3 ( Mlu I) and OXT reverse: 5- gtac- aagctt - ctt gcg cac gtc gag gtc cgg -3 ( Hin dIII)

Also, an Average Change score is computed, based on the average of the individual scores

Restoring NAD+ levels is one of the most promising areas of longevity research, particularly when combined with complementary metabolic compounds

Neuroscience Peptide pathways studied for neurological signaling and biological responses

Preston, J