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Materials and methods Strains Wild type (strain N2), the temperature sensitive-sterile strain TJ1060: spe-9 ( hc88 )

To evaluate the dissolution and release properties of the suppositories, formulations containing 5%, 9%, and 12% water were separately placed in 5 mL of simulated intestinal fluid (SIF, MesGen, Tianjing, China)

The effects vary and should be monitored under medical guidance

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