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et al., 2007

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CRISPR/Cas9 gene targeting gRNAs targeting human LIP and LAP were generated (gRNA 1 5-AGTGGCCAACTTCTACTACG-3, gRNA2 5-CGCTTACCTCGGCTACCAGG-3) and cloned into pSpCas9(BB)-2A-puro (PX459) v2.0 ( Two days after electroporation (Lonza, Fugene Nucleofector Kit, protocol T-20), cells were selected with 1 g/ml puromycin and seeded into clones

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[DOI] [PMC free article] [PubMed] [Google Scholar] Leekumjorn S, Cho HJ, Wu Y, Wright NT, Sum AK, et al