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Crosstalk between HDAC6 and Nox2-based NADPH oxidase mediates HIV-1 Tat-induced pro-inflammatory responses in astrocytes

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Western blot J774 macrophages were cultured in 6-well plates (9.6 cm 2 ), at 1.2 10 6 cells to confluence for 1, 8, and 12 h in RPMI 1640 medium supplemented with 1% FBS, in the presence or absence of 10 M of TMG (SML0244 Sigma), 50 M OSMI-1 (SML1621 Sigma) or 0.5 M Azaserine (A4142 Sigma)

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