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Description
B., Savage, D
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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten min at 95 C
Number of cycles A cycle is a defined treatment period with a protocol length that varies by peptide: CJC/Ipamorelin and Tesamorelin run 12-week (3-month) cycles, the Wolverine Stack (BPC-157/TB-500) runs on a 3-months-on, 2-months-off schedule, and GHK-Cu runs 30-day cycles
