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Nat Methods (2011) 9(1):757

Worms were immediately frozen in briefly in liquid nitrogen, subjected to two or three incomplete freeze/thaw cycles, and then washed with M9 buffer several times to remove the paraformaldehyde solution

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This article discusses the four most common types of melanoma, along with five less common types

Biological or pharmacodynamic half-life the duration of measurable biological effect can differ substantially
