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The cells were treated with blank LPs, free ASX, ASX-LPs, and Lf-ASX-LPs (equivalent to 18 M of ASX) for 6 h, washed with PBS, and incubated in medium containing 0.5 mg/mL MTT for 4 h

The block of the GCLC enzyme may explain the decrease of GSH previously found in OTA-treated cell cultures [29]
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Lamadema N, Burr S, Brewer AC (2019) Dynamic regulation of epigenetic demethylation by oxygen availability and cellular redox

In the first stage, acute reversible immune activation leads to the first seizure, with no permanent brain damage and no obvious brain magnetic resonance imaging changes
