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it's a foundational procedure that dictates the stability, potency, and ultimate reliability of your entire experiment
The bone volume (BV)/total volume (TV) of talus bone was calculated with software (Version: 6.5-3, SCANCO Medical AG, Switzerland) by evaluating and analyzing the three-dimensional region of interest (ROI)
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The column is washed by applying 750 L of buffer PE to and centrifuging for 1 minute
