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Overcoming neuroendocrine and metabolic barriers to puberty: the role of melatonin in advancing puberty in Ewe lambs

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Chen, D., Zhang, X., Li, Z

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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C