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The cell pellet was directly fixed with 70% ice-cold ethanol and stored at -20 C overnight or until analysis

The Journal of Nutrition, Volume 132, Issue 7, July 2002, Pages 18601864

Y., Chen M., Eisenmann E

For instance, gold-based drugs like auranofin show efficacy in treating rheumatoid arthritis and are being studied for their anticancer properties due to their enzyme-inhibitory mechanisms ( auranofin is known to inhibit thioredoxin reductase, an enzyme involved in maintaining cellular redox balance (Tan et al., 2020)

VCN is mainly an inhibitor of the phosphodiesterase-1 enzyme (the enzyme responsible for the degradation of cGMP and cAMP), with a high affinity towards (PDE1A/1B) isozymes 25