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aeruginosa MH602 las B:: gfp (ASV) for LasR analysis, PAO1 rhlA::gfp for RhlR analysis, PAO1 pqs A:: gfp for PqsR analysis, and the clinical isolates DFU-53 (diabetic leg wound ulcer, Liverpool Hospital, NSW, Australia), wound-364,077 (head wound), and the urinary tract isolates UTI-15 and UTI-62 (Royal Prince Alfred Hospital, NSW, Australia)

Many dissociatives have general depressant effects and can produce sedation, respiratory depression, analgesia, anesthesia, and ataxia, as well as cognitive and memory impairment and amnesia

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Using structure-based analyses and cell models, including patient fibroblasts, of this variant, we found that the missense variant destabilized a critical loop, which disrupted the active site and caused a substantial loss of enzymatic function