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After the treatment, cells were incubated with culture medium containing PicoGreen dsDNA Reagent (400-fold dilution, #P11495, Thermo Fisher Scientific) at 37 C for 1 h in the dark

The UV-B caused rapid increase of PcGST1 mRNA preceded the induction of chalcone synthase (CHS), which gene product is produced in the vacuoles protecting plants from UV-B irradiation (Mller-Xing et al., PcGST1 , together with a LUC reporter gene under the control of a CHS promoter, resulted in an earlier UV-dependent CHS : LUC induction

The exquisite immune specificity for beta cells suggests that such cells may already harbour molecular signatures of vulnerability, which may act not only as initiators or conduits for the early immune response but also as amplifiers of ongoing beta cell-directed immune destruction (Kracht et al

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Recent studies have increasingly shown that sncRNA carried by mammalian sperm can transmit paternal environmental influences (such as metabolic diseases, psychological trauma, and exposure to toxic substances) to offspring, affecting their health (Takahashi et al., 2023)