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Tag-Free Pull-Down Systems Tag-free systems mimic endogenous Co-IP , using native or overexpressed bait proteins and specific antibodies

Distinguishing Structural Features of MRP1/ABCC1 Typical mammalian ABC proteins comprise four domains, which are often encoded as a single polypeptide ( e.g

The spacer arm is attached between the ligand and the solid support

And without those bonds, it wont be absorbed into our systems