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Berthon, A
The intein-based system is a well-established example where purified fusion protein cleavage can be induced by thiols such as -mercaptoethanol or 1,4-dithiothreitol (DTT), or by a shift in pH or temperature (Fong et al., 2010, Li, 2011)
The common term alpha-synuclein seed amplification assay (SAA) is now used to apply to both approaches, which have since been replicated by other groups 90,91,92
Yakovlev, L.W
Always consult a qualified healthcare provider before using any peptides
