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The BIOXYTECH assay is based on the SODmediated increase in the rate of autoxidation of 5, 6, 6a, 11b-tetrahydro-3, 9, 10-trihydroxybenzo[c]fluorene R1 in aqueous alkaline solution to yield a chromophore with maximum absorbance at 525 nm

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This reaction generates ATP without the need for the presence of oxygen and thus the expression of PKM2 is increased in many cancer cells

Dipeptidyl peptidase III (DPP3), p62, p21, and the wilms tumor gene on the X chromosome (WTX), Inhibitor of Apoptosis Stimulating Protein of p53 (iASPP) are examples of proteins that function in a non-canonical manner to disrupt the NRF2-KEAP1 association, results in NRF2 stabilization and increased nuclear translocation and activation.10,11 Furthermore, glycogen synthase kinase 3 (GSK-3) phosphorylates NRF2 on serine residues of the DSGIS motif, and forms a phosphodegron, ultimately leading to recruitment of the -TrCP-CUL1-based E3 ubiquitin ligase complex.12 Epigenetic and genetic alterations of KEAP1/NFE2L2 genes in lung cancer patients Genetic alterations such as mutations and copy number variation of key genes encoding KEAP1 and NRF2 proteins have been reported in NSCLC.13 The mutation present in the KELCH domains in the binding site of NRF2 results in the loss of KEAP1 activity