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While the gut bacteria dont produce B12 in amounts significant for human nutrition, they do affect the intestinal environment where B12 absorption occurs

Evidence suggests it may promote neuroprotection, regulate inflammation, and influence mood and cognition, with potential clinical relevance for neurodegenerative and psychiatric disorders

You will also need alcohol swabs for disinfection, bacteriostatic water for reconstitution (if using lyophilized powder), a sterile mixing syringe if reconstituting, and a sharps container for safe needle disposal

William Towers, Olympus, Jr., 9:27.85

How long the effects last depends on your health and how low your B12 levels are