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Leaving the tourniquet in place during the injection can lead to bruising later on, which you definitely dont want

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Early Occlusion-Like Syndrome and BPC 157 Therapy At 30 min after isoprenaline 75 mg/kg s.c., as an occlusion-like syndrome, hearts regularly presented in isoprenaline control rats with congested blood vessels of the myocardium with areas of numerous subendocardial ishemic myocytes, while BPC 157 rats showed no congestion in the heart, only some subendocardial ischemic myocytes and normal T-wave (Figure 1, Figure 3 and Figure 4)

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