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doi: 10.1016/j.molonc.2012.07.003
Key points LABs have been extensively studied and confirmed to alleviate oxidative stress in the body through various mechanisms
You may need to recruit expert consultants in areas where your team lacks experience or collaborate with an academic laboratory
A total of 50 l ChIP lysis buffer containing 5 l mouse monoclonal antibody [anti-Pk (BioRad), anti-Myc (Takara Bio), anti-Flag (Sigma), or anti-RNA polymerase II C-terminal domain (Abcam)] and 15 l Dynabeads protein G (Fisher Scientific) was added to the soluble fraction, and the bead suspension was incubated at 4 C for 2 h with gentle rotation
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