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For the construction of the metE mutant, the AES -which contained a Kan R cassette and the GFP gene optimized for its expression in mycobacteria (eGFP) (Supplementary Note 1)- was synthesized by GenScript
Homocysteine, methylmalonic acid, and/or holotranscobalamin levels as well as a CBC may also be repeated

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Each option has distinct mechanisms, benefits, and limitations
