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The study highlights the in vitro and in vivo stability of a carbamate compared to an ester linker

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Parinandi, The Ohio State University, United States Updates Copyright 2019 Deol, Yang, Morisseau, Hammock and Sladek

For Co-IP/pull-down experiments, pellets obtained from 500 mL expression cultures were resuspended to a calculated OD 600 of 40 in 25 mM TRIS-HCl pH 7, 150 mM KCl, 5% (v/v) glycerol, 10 mM EDTA, and 2% (w/v) n-dodecyl -maltoside (DDM) and incubated for 1 h at 37 C while rotating

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