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The cells were refed once with fresh medium after 4 days and cultured for 3 additional days to generate BM-derived macrophages (BMDM)

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The findings that abemaciclib enhanced tumor cell radiosensitivity, enhanced phosphorylation of gamma-H2AX in combination with radiation, and reduced phosphorylation of p-AKT and p-S6 attenuating PI3K/mTOR signaling as well as alleviating radiation-induced vasculogenesis qualifies it to be a multi-functional radiation modifier

(b) SEC of CydDC H85A in the absence and presence of exogenous heme (applied before the experiment)