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Taken together, our results suggest that colistin-induced mitochondrial depolarization occurs primarily upon opening of the mPTP

Cells were then washed twice with M9, filtered through a 35-m cell strainer, and diluted 1:100 in enrichment media (brain heart infusion [BHI] media supplemented with 500 M l-carnitine)

By integrating current research findings, this review seeks to provide novel directions and strategies for future ASD research and therapy, thereby advancing the field toward more precise, individualized interventions