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All primers were designed for mouse genes and validated for specificity

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The severity of colon mucosal damage was evaluated using the colonic mucosal damage index (CMDI) following the criteria outlined by 2.6 H&E and AB-PAS staining Paraffin-embedded colonic tissue blocks were cut into 4-m-thick sections for H&E staining (Table 2

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The hole is backfilled with the existing soil as well as new composted soil made right here at my farm
