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Here, we present self-assembled querce-tin-Fe3+ nanoparticles (Qu-Fe NPs) for synergetic near-infrared (NIR) light-triggered low-temperature PTT (LTPTT) and glutathione (GSH)-activated CDT

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Alternatively, if your column turns dark brown or black during the purification, this means that youve reduced the nickel ion on the agarose beads, likely due to too much reducing agent such as DTT or TCEP in your purification buffers

This makes it amore advanced and efficient approachcompared to standard glutathione capsules